Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Titration in Nonaqueous Solvents01:16

Titration in Nonaqueous Solvents

Most acid-base titrations are performed in an aqueous medium. In aqueous titrations, water competes with weaker acids or bases for proton donation or acceptance, leading to ambiguous endpoints in the titration curve. Water also affects the partial ionization of weak acids or bases. For example, water accepts a proton from acetic acid to form hydronium and acetate ions. The hydronium ion formed is a stronger acid than acetic acid, and the acetate ion is a stronger base than water. As a result,...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analytical implications of zero-order operation and blazed gratings in chromatographic fluorescence detectors.

Analytica chimica acta·2026
Same author

Hexahydroquinoline Featuring Amide Functionality: A Promising Scaffold With Calcium Channel Blocking Activity.

Drug development research·2026
Same author

A Distinct Anion Separation Approach via Isocratic Liquid Chromatography Coupled with Vacuum Ultraviolet Detection.

Analytical chemistry·2026
Same author

Mirror-Image β-l-Cyclodextrin as a Chiral Pseudophase in Capillary Electrophoresis.

Electrophoresis·2025
Same author

Kinetic investigation on d-amino acid containing peptides and carboxypeptidase Y.

Archives of biochemistry and biophysics·2025
Same author

Back to basic: Using ammonium hydroxide to improve peptide epimer/isomer liquid chromatography separations.

Analytica chimica acta·2025
Same journal

Immunometabolomics Applied to Physical Exercise: Accomplishments and New Directions for Health Improvement.

Annual review of analytical chemistry (Palo Alto, Calif.)·2026
Same journal

Carbon Nanofibers for Mass-Producible Electrochemical Transducers for Point-of-Care Testing.

Annual review of analytical chemistry (Palo Alto, Calif.)·2026
Same journal

Application of Ambient Ionization Mass Spectrometry to the Analysis of <i>Cannabis</i>.

Annual review of analytical chemistry (Palo Alto, Calif.)·2026
Same journal

From Function to Single Cells: Analytical Innovations in Islet Biology and Diabetes Research.

Annual review of analytical chemistry (Palo Alto, Calif.)·2026
Same journal

Quantum Cascade Laser-Based Vibrational Circular Dichroism Imaging for Chiral Biosensing.

Annual review of analytical chemistry (Palo Alto, Calif.)·2026
Same journal

Ion-Ion Chemistry for the Analysis of Biomolecular Ions via Tandem Mass Spectrometry: A Tutorial Review.

Annual review of analytical chemistry (Palo Alto, Calif.)·2026
See all related articles

Related Experiment Video

Updated: Jun 10, 2026

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature
11:04

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature

Published on: December 20, 2016

Ionic liquids in analytical chemistry.

Renee J Soukup-Hein1, Molly M Warnke, Daniel W Armstrong

  • 1Department of Chemistry, Newman University, Wichita, Kansas 67213, USA.

Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|July 20, 2010
PubMed
Summary
This summary is machine-generated.

Ionic liquids (ILs) are revolutionizing analytical chemistry, driving rapid advancements in separations, mass spectrometry, and spectroscopy. New ILs and understanding their properties are key to future innovations in chemical analysis.

More Related Videos

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery
09:44

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Published on: September 26, 2025

From Molecules to Materials: Engineering New Ionic Liquid Crystals Through Halogen Bonding
06:44

From Molecules to Materials: Engineering New Ionic Liquid Crystals Through Halogen Bonding

Published on: March 24, 2018

Related Experiment Videos

Last Updated: Jun 10, 2026

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature
11:04

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature

Published on: December 20, 2016

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery
09:44

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Published on: September 26, 2025

From Molecules to Materials: Engineering New Ionic Liquid Crystals Through Halogen Bonding
06:44

From Molecules to Materials: Engineering New Ionic Liquid Crystals Through Halogen Bonding

Published on: March 24, 2018

Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • The application of ionic liquids (ILs) in analytical chemistry has grown exponentially over the past decade.
  • Early research, once considered niche, is now recognized as foundational to a rapidly expanding field.

Purpose of the Study:

  • To review the historical development and recent progress of ILs in analytical chemistry.
  • To highlight the critical role of novel and multifunctional ILs in advancing analytical techniques.

Main Methods:

  • Literature review focusing on seminal and recent publications in the field.
  • Analysis of trends in separations, mass spectrometry, spectroscopy, and electroanalytical chemistry utilizing ILs.

Main Results:

  • Significant expansion of IL applications across various analytical domains.
  • Identification of new and unique ILs as crucial enablers of recent breakthroughs.
  • Emphasis on the necessity of understanding IL chemical and physical properties.

Conclusions:

  • Ionic liquids are integral to modern analytical chemistry, with ongoing research focused on developing new ILs.
  • Further exploration of IL properties is essential for continued innovation and application in chemical analysis.