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Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Multiplexed spectroscopic detections.

Kyle D Bake¹, David R Walt

¹Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.

Annual Review of Analytical Chemistry (Palo Alto, Calif.)

|July 20, 2010

Summary

This summary is machine-generated.

This review covers multiplexed spectroscopic analysis platforms. It highlights Raman spectroscopy, SPR, and fluorescence for detecting multiple analytes, particularly in biological applications.

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Area of Science:

Analytical Chemistry
Spectroscopy
Biosensing

Background:

Multiplexed spectroscopic analysis enables simultaneous detection of multiple analytes.
Various spectroscopic techniques offer unique advantages for complex sample analysis.
The development of sensitive and selective detection methods is crucial for biological and environmental monitoring.

Purpose of the Study:

To review diverse platforms for multiplexed spectroscopic analysis.
To highlight the application of different spectroscopy types for multiplexed detection.
To discuss the use of cross-reactive sensors and pattern recognition for multi-analyte monitoring.

Main Methods:

Raman spectroscopy
Surface-enhanced Raman spectroscopy (SERS)
Surface plasmon resonance (SPR)
Fluorescence spectroscopy
Cross-reactive sensors
Pattern-recognition algorithms

Main Results:

Platforms utilizing Raman, SERS, SPR, and fluorescence enable multiplexed detection.
Combination of cross-reactive sensors with pattern recognition allows monitoring of multiple analytes in various matrices.
These techniques are effective for detecting biologically relevant analytes.

Conclusions:

Multiplexed spectroscopic techniques offer powerful tools for comprehensive analysis.
The integration of advanced sensors and algorithms enhances detection capabilities.
Significant potential exists for these methods in biological and environmental sensing applications.