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A correlation between polyomavirus JC virus quantification and genotypes in renal transplantation
Wen-Yao Yin1, Ming-Chi Lu, Ming-Che Lee
1Department of General Surgery, Buddhist Dalin Tzu Chi General Hospital, 2 Min-Sheng Road, Dalin town, Chia-Yi, Taiwan. yaowen@mail.tcu.edu.tw
American Journal of Surgery
|July 20, 2010
Summary
John Cunningham virus (JCV) viruria and viral load are significantly higher in transplant patients. Genotyping reveals increased multigenotypes in these patients, indicating a need for comprehensive screening.
Area of Science:
- Virology
- Transplant Immunology
Background:
- John Cunningham virus (JCV) is a polyomavirus that can cause complications in immunocompromised individuals.
- Monitoring viral shedding and genetic diversity is crucial for managing post-transplant outcomes.
Purpose of the Study:
- To investigate the correlation between JCV viral load, viruria, and multigenotypes in renal transplant recipients.
- To assess the diagnostic value of JCV parameters in transplant patients.
Main Methods:
- Urine samples from 60 renal transplant patients and 60 healthy controls were analyzed.
- Quantitative real-time PCR was used to determine JCV viral load.
- Capillary electrophoresis was employed for JCV genotyping.
Main Results:
- Transplant patients exhibited significantly higher rates of JCV viruria and viral load compared to controls (P = .0092, P = .0094).
- Multigenotype detection was significantly more prevalent in transplant patients (5% vs. 0%, P = .0004).
- Higher incidence of multiple genotypes correlated with elevated viral load and was strongly associated with transplant recipients (P = .007).
Conclusions:
- JCV viral load and genotype diversity are critical indicators in transplant patients.
- Comprehensive screening, including genotyping, aids in understanding the immune status.
- This approach can help prevent complications such as polyomavirus nephropathy, infection, and malignancy.
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