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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
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Mg2+ channel selectivity probed by EPR

Yeon-Kyun Shin1

  • 1Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50014, USA. colishin@iastate.edu

Structure (London, England : 1993)
|July 20, 2010
PubMed

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Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...