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Updated: Jun 10, 2026
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
Published on: September 30, 2015
Analysis of a mitochondrial noncoding region for the identification of the most diffused Hypoderma species (Diptera,
Stefania Weigl1, Donato Traversa, Gabriella Testini
1Department of Veterinary Public Health and Animal Sciences, University of Bari, Strada Provinciale per Casamassima km 3, 70010 Valenzano, Bari, Italy.
Abstract:
Hypoderma spp. larvae cause internal myiasis in domestic and wild animals characterized by subcutaneous warbles. Their differentiation is usually performed at species level based on the morphology of third stage larvae. The recent release of the whole mtDNA of Hypoderma lineatum and the retrieval of a 102 bp noncoding region occurring between tRNA(Ser) and NADH dehydrogenase subunit 1 (nd1) genes represented the foundation for this study. The noncoding region and the two flanking mitochondrial genes (i.e., tRNA(Ser) and nd1) of the most diffused Hypoderma spp. (i.e., Hypoderma actaeon, Hypoderma bovis, Hypoderma diana, H. lineatum, Hypodermasinense and Hypoderma tarandi) were analysed. Interspecific variations in amplicon size (from 20 to 102 bp in H. tarandi and H. lineatum, respectively) and nucleotide sequences allowed a genetic discrimination of the examined species providing information instrumental to a rapid molecular identification of Hypoderma spp.
