Adenovirus: a blueprint for non-viral gene delivery

Although adenoviral vectors may not find a direct clinical role in gene therapy, an understanding of the mechanisms of DNA delivery that adenoviruses use is of vital importance to the design of next-generation non-viral gene delivery systems. Adenoviruses overcome a series of biological barriers, including endosomal escape, intracellular trafficking, capsid dissociation, and nuclear import of DNA, to deliver their genome to the host cell nucleus. The understanding of these processes at the molecular level is progressing and is set to inform the design of synthetic gene delivery systems.