Related Experiment Video
Updated: Jun 10, 2026

08:46
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
Published on: August 26, 2020
Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting
Steven Y C Tong1, Rachael A Lilliebridge, Deborah C Holt
1Menzies School of Health Research, Darwin, PO Box 41096 Casuarina, NT 0811, Australia. Steven.tong@menzies.edu.au
Diagnostic Microbiology and Infectious Disease
|July 20, 2010
Abstract:
We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform.
