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Related Experiment Video

Updated: Jun 10, 2026

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
08:38

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Published on: March 19, 2013

Functional skeletal muscle formation with a biologic scaffold.

Jolene E Valentin1, Neill J Turner, Thomas W Gilbert

  • 1Department of Bioengineering, McGowan Institute for Regenerative Medicine, University of Pittsburgh, 450 Technology Drive, Suite 300, Pittsburgh, PA 15219-3130, USA.

Biomaterials
|July 20, 2010
PubMed
Summary

Non-crosslinked biologic scaffolds made from porcine small intestinal submucosa extracellular matrix (SIS-ECM) effectively regenerate functional skeletal muscle tissue. These scaffolds restore muscle contractile force and improve fatigue resistance, outperforming synthetic meshes.

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Biologic scaffolds derived from extracellular matrix (ECM) are utilized for tissue repair, yet their ability to restore in-situ muscle function remains under-evaluated.
  • Most research focuses on structural outcomes, with limited assessment of functional recovery in regenerated skeletal muscle tissue.

Purpose of the Study:

  • To evaluate the in-situ tetanic contractile response and histomorphologic characteristics of skeletal muscle tissue reconstructed with different biomaterials.
  • To compare the functional and structural outcomes of porcine small intestinal submucosa ECM (SIS-ECM) scaffolds against crosslinked SIS-ECM, autologous tissue, and polypropylene mesh.

Main Methods:

  • A rodent abdominal wall model was used to implant four types of tissue reconstruction materials: non-crosslinked SIS-ECM, carbodiimide-crosslinked SIS-ECM, autologous tissue, and polypropylene mesh.

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Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

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Last Updated: Jun 10, 2026

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
08:38

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Published on: March 19, 2013

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

  • Histomorphologic analysis and in-situ tetanic contractile force measurements were performed six months post-surgery to assess tissue regeneration and functionality.

    • Main Results:

    • Remodeled non-crosslinked SIS-ECM scaffolds demonstrated significant skeletal muscle regeneration, achieving maximal contractile force comparable to native tissue and enhanced fatigue resistance.
    • Autologous tissue grafts resulted in a mix of connective and adipose tissue with some skeletal muscle islands, showing native-like fatigue resistance.
    • Crosslinked SIS-ECM and polypropylene mesh induced chronic inflammation and failed to produce significant tetanic force.

    Conclusions:

    • Non-crosslinked xenogeneic SIS scaffolds promote the restoration of functional skeletal muscle tissue with characteristics similar to native muscle.
    • Autologous tissue also supports muscle regeneration, though to a lesser extent than SIS-ECM in this model.
    • Synthetic and crosslinked ECM scaffolds are unsuitable for functional skeletal muscle regeneration due to inflammatory responses and lack of force generation.