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Updated: Jun 10, 2026

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
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Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

Directed 3D cell alignment and elongation in microengineered hydrogels.

Hug Aubin1, Jason W Nichol, Ché B Hutson

  • 1Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Biomaterials
|July 20, 2010
PubMed
Summary
This summary is machine-generated.

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Cells can self-organize in 3D hydrogels to form aligned tissues. This new method uses microengineered gelatin methacrylate (GelMA) hydrogels for precise control over cellular alignment and elongation in tissue engineering.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Organized cellular alignment is crucial for tissue microarchitecture and function.
  • Achieving controlled cellular alignment in 3D engineered tissues is challenging compared to 2D.
  • Existing methods for 3D cellular alignment often rely on external physical stimuli, with limited cell-driven control.

Purpose of the Study:

  • To present a simple, cell-driven method for controlling cellular alignment and elongation in 3D.
  • To demonstrate self-organization of various cell types within microengineered hydrogels.
  • To establish an in vitro model for studying 3D cell and tissue morphogenesis.

Main Methods:

  • Encapsulating fibroblasts, myoblasts, endothelial cells, and cardiac stem cells in 3D gelatin methacrylate (GelMA) hydrogels.

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Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
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Controlled Strain of 3D Hydrogels under Live Microscopy Imaging

Published on: December 4, 2020

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Last Updated: Jun 10, 2026

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel
13:28

Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel

Published on: August 8, 2017

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
07:41

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging

Published on: December 4, 2020

  • Utilizing microengineered hydrogel architectures to confine cells.
  • Observing and analyzing cell self-organization and alignment within the 3D constructs.

    • Main Results:

    • Demonstrated successful control over the alignment and elongation of encapsulated cells.
    • Showcased that cells with intrinsic alignment potential self-organize into functional tissues in vitro when confined.
    • Validated the system's ability to recapitulate native tissue organization in 3D.

    Conclusions:

    • The presented microengineering approach enables precise, cell-driven control of 3D cellular alignment.
    • This method facilitates the creation of engineered tissues with anisotropic function.
    • The system holds significant potential for advancing tissue engineering and in vitro modeling of morphogenesis.