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Isolation of dengue virus with a human promonocyte cell line
1School of Medical Technology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Abstract:
In October-November, 1988 there was an outbreak of dengue fever in the Kaoshiung area of southern Taiwan. We collected 100 serum samples from 96 patients at the onset of their fever for virus cultures and identification. A human promonocyte cell line (HL-CZ) established in our laboratory was used and proved to be susceptible for dengue virus propagation. Type 1 dengue virus in the HL-CZ cell culture was identified by immunofluorescence tests using monoclonal antibodies, and also by hemagglutination tests with goose red blood cells. The density of the virus particles, as measured by sucrose gradient ultracentrifugation, ranged from 1.186 to 1.224 g/ml. The virus yield from this cell culture is comparable with that from the C6/36 mosquito cell line. There was a significant correlation between the antibody responses tested with Western dot blots and hemagglutination inhibition techniques.
Insights
Researchers identified Dengue virus type 1 during a 1988 outbreak in Taiwan. A human cell line (HL-CZ) proved effective for dengue virus propagation and identification, offering results comparable to established mosquito cell lines.
Area of Science:
- Virology
- Infectious Diseases
- Public Health
Background:
- An outbreak of dengue fever occurred in southern Taiwan in October-November 1988.
- Dengue fever is a significant public health concern, necessitating efficient diagnostic and propagation methods.
Purpose of the Study:
- To identify the dengue virus strain responsible for the 1988 outbreak in Kaoshiung, Taiwan.
- To evaluate a human promonocyte cell line (HL-CZ) for dengue virus propagation and characterization.
Main Methods:
- Collected 100 serum samples from 96 patients during the fever onset.
- Utilized a human promonocyte cell line (HL-CZ) for virus culture.
- Identified Dengue virus type 1 using immunofluorescence assays with monoclonal antibodies and hemagglutination tests.
- Determined virus particle density via sucrose gradient ultracentrifugation.
- Correlated antibody responses using Western dot blot and hemagglutination inhibition techniques.
Main Results:
- Successfully propagated Dengue virus type 1 in the HL-CZ human cell line.
- Virus particle density ranged from 1.186 to 1.224 g/ml.
- Virus yield in HL-CZ cells was comparable to the C6/36 mosquito cell line.
- Demonstrated a significant correlation between antibody responses measured by Western dot blot and hemagglutination inhibition.
Conclusions:
- The HL-CZ human cell line is a viable and effective tool for dengue virus propagation and identification.
- The findings provide valuable insights into dengue virus characteristics and host-pathogen interactions during outbreaks.