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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
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Deep Sea Microbial Ecology

The deep ocean and its underlying sediments represent vast, largely unexplored microbial habitats that extend far beyond the sunlit photic zone. The photic (euphotic) zone typically spans the upper ~100–200 meters of pelagic waters in the open ocean, but its depth varies geographically and seasonally, where sufficient light supports photosynthetic life. Below this lies the deep sea, spanning roughly 1000–6000 meters (bathypelagic to abyssal zones), with deeper hadal trenches extending beyond...
Microbes and Other Elemental Cycles01:24

Microbes and Other Elemental Cycles

Microbial activity plays a pivotal role in the biogeochemical cycling of iron and manganese, especially at the redox gradients characteristic of stratified aquatic environments. These cycles are driven by microbial transformations between oxidized and reduced forms of the metals, allowing organisms to exploit them for metabolic energy and structural purposes.Iron Cycling Across Redox GradientsIn neutral, oxygen-rich surface waters, iron is predominantly found in its oxidized, insoluble ferric...
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Methods to Assess Microbial Communities

Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
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Microbial Corrosion

Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...
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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
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Microbial metalloproteomes are largely uncharacterized.

Aleksandar Cvetkovic1, Angeli Lal Menon, Michael P Thorgersen

  • 1Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA.

Nature
|July 20, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a novel metal-based method to identify all metals an organism uses and its metalloproteins. The research reveals that metalloproteomes are more extensive and diverse than previously understood, impacting cell biology and toxicity mechanisms.

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Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

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Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
09:51

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Published on: April 13, 2016

Area of Science:

  • Biochemistry and Molecular Biology
  • Microbiology
  • Proteomics

Background:

  • Metalloproteins are crucial for biological processes, utilizing metal ion cofactors for catalysis, electron transfer, and stability.
  • Current methods struggle to predict an organism's metalloproteome due to diverse and poorly recognized metal coordination sites.
  • Understanding metalloproteomes is essential for insights into cell biology, microbial growth, and toxicity.

Purpose of the Study:

  • To develop and validate a robust, metal-based approach for genome-wide identification of assimilated metals and metalloproteins.
  • To characterize the cytoplasmic metalloproteome of *Pyrococcus furiosus* and compare it with other microorganisms.
  • To reveal the full extent and diversity of metalloproteomes.

Main Methods:

  • Metal-based identification and purification using liquid chromatography.
  • High-throughput tandem mass spectrometry (HT-MS/MS) for protein identification.
  • Inductively coupled plasma mass spectrometry (ICP-MS) for metal quantification.

Main Results:

  • A metal-based strategy identified 158 unpredicted metalloproteins across three microorganisms (*Pyrococcus furiosus*, *Escherichia coli*, *Sulfolobus solfataricus*).
  • Unexpected metals, including lead, manganese, molybdenum, uranium, and vanadium, were found to be assimilated.
  • Novel nickel- and molybdenum-containing proteins were discovered, alongside proteins with misincorporated lead and uranium.

Conclusions:

  • Metalloproteomes are significantly more extensive and diverse than previously recognized.
  • The developed metal-based approach offers a powerful tool for comprehensive metalloproteome analysis.
  • Findings provide critical insights into microbial physiology, metal assimilation, and toxicity mechanisms.