Related Experiment Video

Updated: Jun 10, 2026

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML

P Wimmer1, S Schreiner, R D Everett

  • 1Department of Molecular Virology, Heinrich-Pette-Institute for Experimental Virology and Immunology, Hamburg, Germany.

Oncogene
|July 20, 2010
PubMed

Insights

Human adenovirus E1B-55K protein interacts with the promyelocytic leukaemia (PML) protein, influencing cell transformation. SUMOylation regulates E1B-55K targeting to PML nuclear bodies, impacting p53 tumor suppressor functions.

Area of Science:

  • Molecular biology
  • Virology
  • Cancer research

Background:

  • Human adenovirus E1B-55K is crucial for cell transformation and p53 repression.
  • E1B-55K is modified by the small ubiquitin-related modifier (SUMO)-conjugation system.
  • The precise role of SUMOylation in E1B-55K function remained unclear.

Purpose of the Study:

  • To investigate the biochemical consequences of SUMO1 conjugation on E1B-55K.
  • To determine the interaction between E1B-55K and promyelocytic leukaemia (PML) protein isoforms.
  • To elucidate the role of SUMOylation in regulating E1B-55K localization and function.

Main Methods:

  • Co-immunoprecipitation assays to detect protein interactions.
  • Immunofluorescence microscopy to visualize protein localization.
  • Analysis of viral protein regulation in infected cells.

Main Results:

  • E1B-55K interacts with PML isoforms IV and V, independent of SUMO1.
  • SUMOylation-independent binding to PML-IV directs E1B-55K to PML nuclear bodies (PML-NBs).
  • Viral proteins regulate this interaction in infected cells, suggesting SUMOylation controls E1B-55K targeting to PML-NBs.

Conclusions:

  • SUMOylation regulates E1B-55K targeting to PML-NBs, which are involved in transcriptional regulation, DNA repair, and apoptosis.
  • SUMO1-dependent modulation of E1B-55K and PML-IV interaction plays a role in adenovirus-mediated cell transformation by affecting p53 function.

More Related Videos

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...