Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Breaking the immune "cold niche" in bone metastasis: core mechanisms of the multidimensional interwoven regulatory network and precision breakthrough strategies.

Molecular cancer·2026
Same author

Preserving a Kinetically-Metastable Nanophase by Limited Calcination for High-Performance Protonic Ceramic Cells.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Image stitching for probe-based confocal laser endomicroscopy via a motion consistency constraint.

Biomedical optics express·2026
Same author

A thalamus-brainstem attractor network drives history-biased decisions.

Nature·2026
Same author

Transcriptome-wide association study of prostate cancer in the Chinese population.

Asian journal of urology·2026
Same author

Three-dimensional resolution enhancement of two-photon microscopy by combining point spread function engineering and multi-image deconvolution.

Optics letters·2026

Related Experiment Video

Updated: Jun 10, 2026

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain
08:26

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Published on: January 13, 2022

Pulse compression in two-photon excitation fluorescence microscopy.

Xiaobao Liang1, Wenyan Hu, Ling Fu

  • 1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China.

Optics Express
|July 20, 2010
PubMed
Summary

Shorter laser pulses enhance two-photon microscopy signals. Researchers compressed pulses using photonic crystal fiber, achieving a 5.6x increase in NAD(P)H fluorescence for improved disease diagnosis.

More Related Videos

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
08:39

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jun 10, 2026

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain
08:26

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Published on: January 13, 2022

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
08:39

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

Area of Science:

  • Biophysics
  • Optical Engineering
  • Medical Imaging

Background:

  • Two-photon excitation fluorescence microscopy (TPEFM) is a powerful imaging technique.
  • Improving signal-to-noise ratio in TPEFM is crucial for enhanced sensitivity and diagnostic capabilities.
  • Shorter laser pulses offer a practical method to boost signal intensity in TPEFM.

Purpose of the Study:

  • To investigate pulse compression for TPEFM using a Ti:Sapphire laser and photonic crystal fiber.
  • To optimize compressor performance by analyzing fiber parameters, transmitted power, and grating-induced group-delay dispersion.
  • To demonstrate the practical application of compressed pulses for enhanced cellular imaging and disease diagnosis.

Main Methods:

  • Theoretical and experimental analysis of pulse compression using a highly nonlinear photonic crystal fiber.
  • Optimization of fiber length, zero dispersion wavelength, transmitted power, and grating dispersion.
  • Integration of the pulse compressor with a TPEFM system.
  • Measurement of autofluorescence intensity of NAD(P)H in Nasopharyngeal carcinoma cells.

Main Results:

  • Experimental demonstration of pulse compression from 94 fs to 23.6 fs at 790 nm using a 20-mm photonic crystal fiber (zero dispersion wavelength 850 nm).
  • Verification of simulation results through experimental data.
  • Achieved a 5.6-fold increase in NAD(P)H autofluorescence intensity in Nasopharyngeal carcinoma cells.
  • Demonstrated the effectiveness of the optimized pulse compressor in enhancing TPEFM signals.

Conclusions:

  • Pulse compression using photonic crystal fiber is an effective strategy to improve TPEFM signal intensity.
  • The developed pulse compression system shows significant potential for enhanced imaging and sensing applications in disease diagnosis.
  • This technique can lead to more sensitive detection and characterization of biological samples and disease markers.