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Hideaki Takashima1, Takeshi Asai, Kiyota Toubaru

  • 1Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0020, Japan.

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|July 20, 2010
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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...