Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Affinity and Avidity01:41

Affinity and Avidity

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Virtual special issue for HPLC 2024.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same author

Studies and analysis of drug-target interactions by affinity chromatography and related techniques: A review.

Journal of pharmaceutical analysis·2026
Same author

Screening of binding by antidiabetic drugs to normal vs AGE-modified human serum albumin through covalent immobilization and microscale affinity chromatography.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same author

Microscale Affinity Chromatography for Biointeraction Analysis: Strategies, Principles and Applications.

Journal of separation science·2026
Same author

Chromatographic-Based Binding and Thermodynamic Studies of Antibiotic Micropollutants with Humic Acid Using Affinity Microcolumns.

Journal of separation science·2026
Same author

Refreshing the Aims and Scope of the Journal of Chromatography B.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2025

Related Experiment Video

Updated: Jun 10, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Immunoaffinity chromatography: an introduction to applications and recent developments.

Annette C Moser1, David S Hage

  • 1Chemistry Department, University of Nebraska at Kearney, Kearney, NE 68849-1150, USA. moserac@unk.edu

Bioanalysis
|July 20, 2010
PubMed
Summary
This summary is machine-generated.

Immunoaffinity chromatography (IAC) offers specific target analysis and purification using antibodies. This review covers its principles, antibody properties, and diverse applications, including hyphenation with CE and MS.

More Related Videos

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
10:33

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

Protein Complex Affinity Capture from Cryomilled Mammalian Cells
10:37

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

Published on: December 9, 2016

Related Experiment Videos

Last Updated: Jun 10, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
10:33

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

Protein Complex Affinity Capture from Cryomilled Mammalian Cells
10:37

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

Published on: December 9, 2016

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Immunology

Background:

  • Immunoaffinity chromatography (IAC) leverages specific antibody-antigen interactions for analytical and purification purposes.
  • It combines liquid chromatography (LC) with the high specificity of antibodies or related binding agents.
  • IAC is valuable for assays, analyte purification, and concentration before further analysis.

Purpose of the Study:

  • To review the history, principles, and formats of Immunoaffinity Chromatography (IAC).
  • To provide an overview of antibody properties, production, and immobilization techniques for IAC.
  • To discuss various applications and recent developments in IAC.

Main Methods:

  • Review of literature on Immunoaffinity Chromatography (IAC) principles and applications.
  • Discussion of antibody properties, production, and immobilization strategies.
  • Analysis of support materials, application/elution conditions, and integration with other techniques.

Main Results:

  • IAC principles, antibody characteristics, and immobilization methods are detailed.
  • Various IAC formats and applications like purification, immunodepletion, and assays are presented.
  • Recent advancements include coupling IAC with CE/MS and microanalytical systems.

Conclusions:

  • Immunoaffinity Chromatography (IAC) is a versatile technique for specific analyte isolation and analysis.
  • The review highlights the broad applicability and ongoing evolution of IAC in analytical science.
  • IAC continues to advance with hyphenation to other techniques and miniaturization.