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Updated: Jun 10, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Redesign the α/β fold to enhance the stability of mannanase Man23 from Bacillus subtilis
Hai-Yan Zhou1, Hong-Ya Pan, Li-Qun Rao
1The Center of Analysis and Measurement, Hunan Agricultural University, Changsha, China.
Abstract:
In this work, we engineered the α/β fold of mannanase Man23 based on its molecular structure analysis to obtain more stable variants. By introducing 31 single-site mutations in the α/β fold and shuffling them, the incorporation of four mutations (K178R, K207R, N340R, and S354R) displayed a good balance between high activity and stability at higher temperature and broader pH. This quartet variant was characterized by an almost threefold increased activity and a sevenfold increased stability compared to native mannanase Man23. Our results suggest that such work is safe to increase our target protein stability with no loss of activity.
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