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Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
Published on: October 18, 2019
[Preliminary identification of clinically significant Clostridium species]
1Central Laboratories, The Laboratory of Bacteriology, Ceské Budejovice, Czech Republic. balejova@nemcb.cz
Abstract:
Preliminary identification of clinically significant Clostridium spp. is based on evaluating their microscopic and macroscopic morphology, Gram staining (Gram stain-positive structure of the bacterial wall), positive production of lecithinase, lipase and proteolytic activity on egg yolk agar, and simple chemical tests. If this preliminary identification is not sufficient, biochemical identification is performed, along with 16S-rRNA sequencing of the bacterial genome. The article comments on options of preliminary identification of clinically significant Clostridium spp.
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