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Published on: June 24, 2025
Hospital acquired infections in a medical intensive care unit
1Department of Pathology, Rawalpindi Medical College, Rawalpindi. naeeakh@yahoo.com
Objective:
To determine the frequency of nosocomial infections and causative organisms in medical intensive care unit (ICU) patients and antimicrobial susceptibility pattern of the isolates.
Study Design:
A cross-sectional study.
Place And Duration Of Study:
The Holy Family Hospital, Rawalpindi, during the period of May 2007 to April 2008.
Methodology:
Clinical samples from patients having any signs of site-specific infections or fever appearing anytime after 48 hours of admission into ICU were collected. The samples were cultured onto suitable culture media and bacterial isolates were identified using standard biochemical methods. Antimicrobial susceptibility testing to conventional and newer antibiotics was performed on Mueller Hinton agar using disc diffusion method. Frequency percentages were determined.
Results:
Bacteria or Candida spp. were isolated from 269/440 (60.1%) samples. The most frequent site of infection was respiratory tract (47.95%) followed by urinary tract (25.3%). Pseudomonas (P.) aeruginosa, Klebsiella (K.) pneumoniae, Escherichia (E.) coli and Candida spp. were the commonest organisms. The isolation rate of Gram-positive bacteria was relatively low. Majority (> 50%) of the Gram-negative isolates were resistant to many of the antibiotics tested. Relatively low resistance was only observed against amikacin (21.3%) and imipenem (26.1%). Majority (> 60%) of Gram-negative isolates were resistant to cefotaxime, ceftriaxone and ceftazidime. The isolates showed high resistance to ofloxacin (65.9%) and ciprofloxacin (73.9%).
Conclusion:
The high frequency of HAIs and antibiotic resistance rate, suggests that more strict infection control practices along with prescription of antibiotics after antibiotic susceptibility testing should be implemented to limit the emergence of antibiotic resistant organisms.
Insights
Nosocomial infections are common in intensive care units (ICUs), with respiratory and urinary tracts most affected. High antibiotic resistance rates necessitate stricter infection control and susceptibility testing for effective treatment.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Hospital Epidemiology
Background:
- Healthcare-associated infections (HAIs) pose a significant challenge in intensive care units (ICUs).
- Understanding the spectrum of pathogens and their antimicrobial susceptibility is crucial for effective patient management.
Purpose of the Study:
- To determine the incidence of nosocomial infections in medical ICU patients.
- To identify causative organisms and their antimicrobial susceptibility patterns.
Main Methods:
- A cross-sectional study was conducted at Holy Family Hospital, Rawalpindi.
- Clinical samples were collected from ICU patients with signs of infection.
- Standard microbiological techniques and antimicrobial susceptibility testing (disc diffusion) were employed.
Main Results:
- 60.1% of samples yielded bacterial or Candida spp. isolates.
- Respiratory tract infections (47.95%) and urinary tract infections (25.3%) were most common.
- Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, and Candida spp. were prevalent; high resistance to common antibiotics was observed, except for amikacin and imipenem.
Conclusions:
- High rates of HAIs and antibiotic resistance were found in the medical ICU.
- Implementation of strict infection control practices is recommended.
- Antibiotic prescription should be guided by susceptibility testing to combat emerging resistance.
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