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Related Concept Videos

Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
Solubility03:00

Solubility

Solution, Solubility, and Solubility Equilibrium
A solution is a homogeneous mixture composed of a solvent, the major component, and a solute, the minor component. The physical state of a solution—solid, liquid, or gas—is typically the same as that of the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
In a solution, the solute particles (molecules, atoms, and/or ions)...
The Colloidal State01:29

The Colloidal State

The formation of a colloidal system is exemplified by an aqueous solution containing Cl− ions is introduced to another containing Ag+ ions, resulting in the precipitation of solid AgCl as extremely tiny crystals. Instead of settling out as a filterable precipitate, these crystals remain suspended in the liquid, showcasing a colloidal system.A colloidal system involves colloidal particles within the approximate range of 1 to 1000 nm in at least one dimension, dispersed in a medium called the...
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

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Related Experiment Video

Updated: Jun 10, 2026

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
07:26

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

Published on: January 9, 2012

Bicosomes: bicelles in dilute systems.

Gelen Rodríguez1, Guadalupe Soria, Elisenda Coll

  • 1Departament de Tecnologia Química i de Tensioactius, Institut de Química Avançada de Catalunya, Consejo Superior de Investigaciones Científicas, Barcelona, Spain.

Biophysical Journal
|July 21, 2010
PubMed
Summary
This summary is machine-generated.

Encapsulating bicelles (lipid nanostructures) within liposomes creates bicosomes, preserving their discoidal shape in dilute solutions. This novel approach enhances bicelle stability and enables safe in vivo application.

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Biophysics

Background:

  • Bicelles are discoidal phospholipid nanostructures crucial in various biological and materials science applications.
  • Bicelles exhibit morphological instability, increasing in size and changing shape under dilute conditions, limiting their utility.
  • A method is needed to maintain the precise discoidal morphology of bicelles in aqueous environments.

Purpose of the Study:

  • To develop a strategy for preserving the discoidal morphology of bicelles in high water content environments.
  • To evaluate the stability and in vivo safety of encapsulated bicelles (bicosomes).

Main Methods:

  • Bicelles were formed under concentrated conditions and subsequently encapsulated within liposomes, creating bicosomes.
  • Dynamic light-scattering spectroscopy and cryo-transmission electron microscopy were used to characterize bicelle morphology before and after dilution.
  • Bicosomes and free bicelles containing gadodiamide were injected into rat brain ventricles, with imaging via magnetic resonance imaging.

Main Results:

  • Encapsulation within liposomes successfully protected bicelles from morphological changes upon dilution.
  • Free bicelles altered in size and shape in dilute conditions, while encapsulated bicelles remained stable.
  • Bicosomes demonstrated in vivo biocompatibility and safety in rat models, unlike free bicelles.

Conclusions:

  • Bicosomes effectively preserve the discoidal morphology of bicelles in dilute solutions.
  • Liposome encapsulation provides a robust method for stabilizing nanostructures.
  • Bicosomes represent a promising platform for the controlled delivery and application of bicelles in biological systems.