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Athymic rats in preclinical immunodiagnostic studies
R T Storeng1, I Kjønniksen, A Pihl
1Department of Tumor Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo.
Anticancer Research
|March 1, 1991
Summary
Nude rat xenografts are useful for cancer studies, but antibody behavior differs from nude mice. Results in nude mice cannot always be extrapolated to nude rats for immunolocalization research.
Area of Science:
- Oncology
- Immunology
- Preclinical Research
Background:
- Nude rat xenografts are utilized for immunolocalization studies.
- The monoclonal antibody 9.2.27 targets melanomas and osteosarcomas.
Purpose of the Study:
- To investigate the utility of nude rat xenograft systems for immunolocalization.
- To compare antibody behavior in nude rats versus nude mice.
Main Methods:
- Established human melanoma (LOX, FEMX-I) and osteosarcoma (OHSX) xenografts in nude rats.
- Administered radiolabeled 9.2.27 F(ab')2 fragments intravenously.
- Conducted biodistribution and immunoscintigraphic studies.
Main Results:
- Antibody 9.2.27 concentrated in LOX and OHSX xenografts (tumor to blood ratio up to 30).
- Antibody failed to concentrate in FEMX-I xenografts, unlike in mice.
- Immunoscintigraphy visualized subcutaneous tumors well, but lung colonies were less clear.
- Radiography outperformed immunoscintigraphy for detecting lung tumors.
Conclusions:
- Nude rat xenografts are valuable for preclinical cancer research.
- Results from nude mouse models are not always directly applicable to nude rats.
- Tumor site influences antibody homing and detection efficacy.