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Updated: Jun 10, 2026

In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Trigeminal Ganglia
Published on: August 1, 2025
Facilitation of Ih channels by P2Y1 receptors activation in Mesencephalic trigeminal neurons
Wei Huang1, Yun Xiu, Jun-an Yan
1Department of Neurobiology, College of Basic Medical Sciences, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China.
Abstract:
P2Y(1) receptors, a subset of G-protein coupled receptors, have been shown to participate in sensory transduction in the periphery nervous system. However, little is known about their sensory function in the central nervous system. Here, by using immunohistochemistry, we showed that P2Y(1) receptors are predominantly localized in the somata of Mesencephalic trigeminal neurons (Mes V neurons), the primary sensory neurons in brainstem. Whole-cell voltage-clamp recording revealed that ADP-beta-S, a P2Y receptor agonist, enhanced the activity of hyperpolarization-activated cation channels (Ih channels) in Mes V neurons and that the activity-enhancing effect of ADP-beta-S could be blocked by a specific P2Y(1) receptor antagonist, MRS 2179. Taken together, these results suggested a possible role of P2Y(1) receptors in the information transduction of central sensory neurons through regulating Ih channel activities.
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