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Updated: Jun 10, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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Published on: July 4, 2018

Mast cell function is not altered by Coronin-1A deficiency.

Sanja Arandjelovic1, Dilki Wickramarachchi, Saskia Hemmers

  • 1Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92093, USA.

Journal of Leukocyte Biology
|July 21, 2010
PubMed
Summary

Coronin-1A protein inactivation in mice enhances lupus resistance but impairs T cell function. However, mast cell functions, including degranulation and cytokine secretion, remain unaffected, indicating Coronin-1A is not essential for mast cells.

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Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Coronin-1A is a WD repeat protein regulating F-actin dynamics and Ca2+ signaling, highly expressed in hematopoietic cells.
  • Inactivation of Coronin-1A (Coro1a) in mice confers resistance to lupus erythematosus but impairs peripheral T cell functions.
  • The role of Coronin-1A in mast cell biology remains largely unexplored.

Purpose of the Study:

  • To investigate the functional role of Coronin-1A in mast cell degranulation, cytokine secretion, and migration.
  • To determine if Coronin-1A is essential for mast cell viability and normal development.
  • To compare mast cell responses in Coronin-1A deficient mice with wild-type (WT) mice.

Main Methods:

  • In vitro differentiation of mast cells from Coro1a(Lmb3) mutant mice and WT littermates.
  • Assessment of mast cell viability, development, and F-actin levels.
  • Functional assays including degranulation, cytokine secretion (e.g., TNF-α, IL-6), chemotactic migration, and FcεRI-mediated Ca2+ flux.
  • Analysis of Coronin-1B and Coronin-1C expression in mast cells and other immune cells.

Main Results:

  • In vitro-differentiated mast cells from Coro1a(Lmb3) mice were viable, developed normally, and exhibited normal degranulation, cytokine secretion, and chemotactic migration.
  • Increased F-actin levels in Coro1a(Lmb3) mast cells did not impair their function.
  • Calcium (Ca2+) flux in response to FcεRI stimulation was unaffected in Coro1a(Lmb3) mast cells.
  • Coro1a(Lmb3) mice displayed comparable in vivo mast cell responses to WT mice.
  • Coronin-1B and Coronin-1C expression was not upregulated in Coro1a(Lmb3) mast cells.

Conclusions:

  • Coronin-1A activity is not required for the normal development and function of mast cells.
  • Mast cells can maintain normal F-actin dynamics and Ca2+ signaling essential for their function even in the absence of functional Coronin-1A.
  • These findings highlight a cell-type-specific role for Coronin-1A within the hematopoietic system.