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Head Implants for the Neuroimaging of Awake, Head-Fixed Rats07:01

Head Implants for the Neuroimaging of Awake, Head-Fixed Rats

A detailed new procedure for functional imaging of awake, head-fixed rats is...
2.9K
Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis07:07

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

This study describes a new method of isolating murine brown adipocytes for gene and protein expression...
6.9K
Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice09:00

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

This report describes a protocol for the simultaneous isolation of primary brown and white preadipocytes from newborn mice. Isolated cells can be grown in culture and induced to differentiate into fully mature white and brown adipocytes. The method enables genetic, molecular, and functional characterization of primary fat cells in...
13.4K
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue07:50

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Here we describe a protocol for efficient chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-seq) of brown adipose tissue (BAT) isolated from a mouse. This protocol is suitable for both mapping histone modifications and investigating genome-wide localization of non-histone proteins of interest in...
8.5K
White and Brown Adipose Depot Collection from Mouse Pup: A Surgical Procedure to Harvest the White Adipose Tissue and Brown Adipose Tissue from Mouse Pup03:28

White and Brown Adipose Depot Collection from Mouse Pup: A Surgical Procedure to Harvest the White Adipose Tissue and Brown Adipose Tissue from Mouse Pup

In this video, we demonstrate the surgical procedure to isolate the subcutaneous white adipose tissue and interscapular brown adipose tissue from a newborn...
4.7K
Muscles that Move the Head01:19

Muscles that Move the Head

The muscles that move the head are a dynamic and complex group of structures that work together to facilitate a wide range of head movements, including rotation, flexion, extension, and lateral bending.
The bilateral sternocleidomastoid, or SCM, and the suprahyoid and infrahyoid muscles are significant head flexors. The SCM muscles originate at the sternum and clavicle and attach to the mastoid process of the temporal bone. The SCM contracts bilaterally to bend the head forward, whereas...
5.6K