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In vitro cyclooxygenase activity assay in tissue homogenates

Samir S Ayoub1

  • 1Centre for Biochemical Pharmacology, Experimental Pathology Group, St. Bart's and the London School of Medicine and Dentistry, William Harvey Research Institute, Queen Mary University of London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|July 21, 2010
PubMed

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Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
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