Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combination of degrading with non-degrading endophytic bacteria synergistically enhances rice resistance to chlorpyrifos stress.

Journal of advanced research·2026
Same author

CRISPR Cas9 mediated genome editing to induce apoptosis a new strategy to tackle cancer.

Nucleosides, nucleotides & nucleic acids·2026
Same author

Quantifying Regional and Age-Dependent Microstructural Changes in Porcine Ventricles from Neonatal to Adulthood Using DT-MRI and TPEF-SHG Microscopy.

Annals of biomedical engineering·2026
Same author

Challenges in translating AI-driven ASD/ADHD diagnosis: A methodological systematic review.

International journal of medical informatics·2026
Same author

From Hotspots to Action: Spatial Risk and Habitat Priorities for Human-Macaque Coexistence in the Hindu Raj Mountains, Pakistan.

American journal of primatology·2026
Same author

Alternative splicing generates a novel CARD9 isoform.

Biochimie·2026

Related Experiment Video

Updated: Jun 10, 2026

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Structural and functional analysis of human prostatic acid phosphatase.

Md Imtaiyaz Hassan1, Afnan Aijaz, Faizan Ahmad

  • 1Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.

Expert Review of Anticancer Therapy
|July 22, 2010
PubMed
Summary

Prostatic acid phosphatase (PAP) is a key biomarker for prostate cancer and plays a role in fertilization. This review details PAP

More Related Videos

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

Related Experiment Videos

Last Updated: Jun 10, 2026

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

Area of Science:

  • Biochemistry
  • Oncology
  • Molecular Biology

Background:

  • Prostatic acid phosphatase (PAP) is abundant in prostate secretions.
  • PAP is a significant biomarker for prostate carcinoma.
  • PAP may have a role in human fertilization.

Purpose of the Study:

  • To provide a comprehensive overview of prostatic acid phosphatase (PAP).
  • To discuss PAP's gene regulation, structure, and functions.
  • To explore PAP's role in prostate cancer progression and evolution.

Main Methods:

  • Literature review of existing research on PAP.
  • Analysis of gene regulation, protein structure, and catalytic mechanisms.
  • Examination of evolutionary aspects and clinical relevance.

Main Results:

  • PAP exhibits phosphatase activity, crucial for dephosphorylating macromolecules.
  • Catalytic residues (His12 and Asp258) are key to PAP's enzymatic function.
  • PAP's established role as a prostate cancer biomarker is highlighted.

Conclusions:

  • Prostatic acid phosphatase (PAP) is a multifaceted protein with implications in cancer and reproduction.
  • Understanding PAP's features is vital for prostate pathophysiology research.
  • This review consolidates current knowledge on PAP for scientific advancement.