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Ryota Suzuki1, Masaru Ihira, Yoshihiko Enomoto

  • 1Department of Pharmacy, Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Japan.

Microbiology and Immunology
|July 22, 2010
PubMed

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PCR01:32

PCR

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...