Related Experiment Video
Updated: Jun 10, 2026
Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
Published on: August 31, 2016
The enrichment of trophoblast giant cells from mid-gestation bovine placentae using fluorescence-activated cell
K A Pennington1, L J Oliveira, Q E Yang
1Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, FL 32611, USA.
Abstract:
The objective of this work was to determine if fluorescence-activated cell sorting (FACS) can be used to isolate trophoblast giant cells (TGCs) from bovine placentae. Cotyledons were harvested, minced and digested with dispase/pancreatin. Tissue homogenates were incubated with Vybrant Dye Cycle-Green. FACS was completed, and cells with DNA content 2- to 4-times greater than cells within the diploid peak contained between 65 and 84% TGCs. The relative abundance of CSH1 and PAG1 transcripts were greater (P < 0.05) in TGC-enriched fractions than cells within the diploid peak. These observations indicate that FACS can effectively enrich TGCs from bovine placentae.
