Multicolor fluorescence RNA in situ hybridization of Drosophila brain tissue

RNA in situ hybridization is a useful method for determining the transcriptional expression pattern of a gene when antibodies are not available. Using this technique, it is possible to assay the expression of multiple RNA species using distinct labels on RNA probes, or simultaneously examine RNA and protein localization within larval tissues. This protocol describes RNA in situ hybridization of Drosophila brain tissue. It utilizes a fluorophore-conjugated tyramide that is easily made in the laboratory for a fraction of the cost of the commercially produced product.