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Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens
Published on: April 19, 2011
Simplified procedure for transient transformation of plant protoplasts using polyethylene glycol treatment
L A Lyznik1, J Y Peng, T K Hodges
1Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907.
Biotechniques
|March 1, 1991
Abstract:
A modification of the polyethylene glycol-mediated transformation procedure which eliminates the manual polyethylene glycol dilution step is presented. A transformation mixture of protoplasts, DNA and polyethylene glycol was plated directly onto agarose blocks after incubation. The procedure was simple and fast, thereby suitable for screening the gene activity of large numbers of plasmid constructions. It has been tested for both maize and rice protoplasts.
