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Updated: Jun 10, 2026

Modification of a Colliculo-thalamocortical Mouse Brain Slice, Incorporating 3-D printing of Chamber Components and Multi-scale Optical Imaging
Published on: September 18, 2015
Specific and nonspecific thalamocortical connectivity in the auditory and somatosensory thalamocortical slices
Brian B Theyel1, Charles C Lee, S Murray Sherman
1Department of Neurobiology, University of Chicago, Chicago, Illinois 60637, USA. btheyel@uchicago.edu
Abstract:
Two classes of thalamic nuclei project to either middle layers or upper layers, including layer 1, of the neocortex, and are referred to as 'specific' and 'nonspecific' nuclei, respectively. The electrophysiological properties of the nonspecific nuclei have not been investigated, largely because of the paucity of in vitro slice preparations containing intact nonspecific pathways. In this study, we used flavoprotein autofluorescence imaging to show intact thalamocortical connectivity of nonspecific nuclei in slice preparations of the somatosensory and auditory systems. These preparations will enable the elucidation of electrophysiological properties of nonspecific pathways.
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