Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-enhanced fluorescent biosensors using metal-coated piezoelectric nanoimprinted substrates.

Physical chemistry chemical physics : PCCP·2026
Same author

Piezoelectric-Driven Amplification of Plasmon-Enhanced Fluorescence for Advanced Sensing Applications.

ACS applied materials & interfaces·2025
Same author

Nanoimprinted PVDF-MWCNT Composites with Silver Coating as a Label-Free Plasmonic Platform for Ultrasensitive Detection.

ACS applied nano materials·2025
Same author

Flexible nanoimprinted substrate integrating piezoelectric potential and photonic-plasmonic resonances.

Nanoscale advances·2025
Same author

p-Type Organic Semiconductor-Metal Nanoparticle Hybrid Film for the Enhancement of Raman and Fluorescence Detection.

The journal of physical chemistry. C, Nanomaterials and interfaces·2025
Same author

Metal-Free Peptide Semiconductor-Enhanced Raman Scattering.

Nano letters·2024

Related Experiment Video

Updated: Jun 10, 2026

Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
06:54

Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems

Published on: June 23, 2023

Nanoscale optical imaging by atomic force infrared microscopy.

James H Rice1

  • 1School of Physics, University College Dublin, Belfield, Dublin, Ireland. james.rice@ucd.ie

Nanoscale
|July 22, 2010
PubMed
Summary

Atomic force infrared microscopy advances nanoscale infrared absorption imaging. This technique is applied to biological and functional materials, including cellular systems in water.

Area of Science:

  • Spectroscopy and microscopy
  • Materials science
  • Biophysics

Background:

  • Atomic force microscopy (AFM) provides high-resolution surface topography.
  • Infrared (IR) spectroscopy probes molecular vibrations and chemical composition.
  • Integrating IR with AFM enables nanoscale chemical mapping.

Purpose of the Study:

  • To review the progress of atomic force infrared microscopy (AFIRM).
  • To discuss the methodology and applications of AFIRM.
  • To highlight AFIRM's use in imaging biological and functional materials.

Main Methods:

  • Atomic force infrared microscopy (AFIRM) combines AFM with IR spectroscopy.
  • AFIRM utilizes a sharp AFM tip to locally enhance the IR absorption signal.

More Related Videos

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

Related Experiment Videos

Last Updated: Jun 10, 2026

Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
06:54

Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems

Published on: June 23, 2023

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

  • This allows for chemical identification and imaging at the nanoscale.

    • Main Results:

    • AFIRM enables high-resolution infrared absorption imaging at the nanoscale.
    • The technique has been successfully applied to various biological and functional materials.
    • AFIRM can image cellular systems in aqueous environments, overcoming previous limitations.

    Conclusions:

    • Atomic force infrared microscopy is a powerful emerging technique for nanoscale chemical analysis.
    • Its ability to image in aqueous environments expands its utility for biological studies.
    • Continued development promises broader applications in materials science and cell biology.