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Updated: Jun 10, 2026

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
Luminescence resonance energy transfer from an upconverting nanoparticle to a fluorescent phycobiliprotein
Fiorenzo Vetrone1, Rafik Naccache, Christopher G Morgan
1Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke St. W., Montreal, QC H4B 1R6, Canada.
Abstract:
Water dispersible upconverting polyethylenimine (PEI)-capped NaYF(4) nanoparticles co-doped with trivalent erbium (Er(3+)) and ytterbium (Yb(3+)) were prepared via solvothermal synthesis with an 18 nm average particle diameter. These upconverting nanoparticles can be used to sensitize a light-harvesting phycobiliprotein (R-Phycoerythrin) via luminescence resonance energy transfer (LRET).
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