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Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

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Published on: March 9, 2018

Progress in SIFT-MS: breath analysis and other applications.

Patrik Spaněl¹, David Smith

¹J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejškova 3, 182 23, Prague 8, Czech Republic. patrik.spanel@jh-inst.cas.cz

Mass Spectrometry Reviews

|July 22, 2010

Summary

Selected ion flow tube mass spectrometry (SIFT-MS) has evolved into a sensitive, on-line breath analysis tool. The Profile 3 instrument enables real-time quantification of metabolites and volatile compounds for various health applications.

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Area of Science:

Analytical Chemistry
Mass Spectrometry
Biomedical Engineering

Background:

Selected ion flow tube mass spectrometry (SIFT-MS) has undergone significant development from large-scale instruments to compact, transportable Profile 3 systems.
Understanding the underlying physics and engineering is crucial for designing and building effective SIFT-MS instruments.

Purpose of the Study:

To describe the evolution and capabilities of SIFT-MS, particularly the Profile 3 instrument.
To illustrate the versatility and sensitivity of SIFT-MS for on-line analyses.
To highlight recent advancements and future prospects in SIFT-MS technology.

Main Methods:

Utilized H(3)O(+), NO(+), and O(2)(+)· precursor ions for SIFT-MS analysis.
Emphasized the importance of accounting for differential ionic diffusion and mass discrimination in analytical algorithms.
Conducted pilot studies for on-line breath, urine, and skin volatile compound analysis.

Main Results:

Demonstrated real-time quantification of breath metabolites in healthy adults and children, identifying systemic and orally-generated compounds.
Showcased SIFT-MS's ability to detect drug-induced metabolite changes, identify HCN as a Pseudomonas marker, and analyze volatile compounds in urine and skin.
Presented recent developments including CO2 quantification and hyphenation with GC and ATD.

Conclusions:

SIFT-MS, especially the Profile 3 instrument, is a versatile and sensitive technique for on-line trace gas analysis.
The technology has proven effective in various pilot studies, offering insights into human metabolism and disease markers.
Ongoing developments promise expanded applications and improved analytical performance for SIFT-MS.