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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 10, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

[Atomic force microscopy involved in protein study].

Zhengjian Lu1, Guoping Chen, Jianhua Wang

  • 1College of Bioengineering, Chongqing University, Chongqing 400044, China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi
|July 24, 2010
PubMed
Summary
This summary is machine-generated.

Atomic force microscopy (AFM) offers high-resolution imaging and analysis of proteins under physiological conditions. This nanotechnology enables detailed studies of protein behavior, from folding to assembly and molecular recognition.

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Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
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Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

Related Experiment Videos

Last Updated: Jun 10, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
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Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Biology

Context:

  • Atomic force microscopy (AFM) is an advanced nano microscopic technology.
  • AFM provides sub-nanoscale resolution, enabling detailed molecular-level investigations.
  • It operates without extensive sample preparation and allows real-time studies in physiological and aqueous environments.

Purpose:

  • To introduce the principles and operational modes of Atomic Force Microscopy.
  • To highlight the significant advancements and applications of AFM in protein studies.
  • To explore AFM's utility in various aspects of protein research, including imaging, adsorption, folding-unfolding dynamics, assembly, and single-molecule recognition.

Summary:

  • This paper details the fundamental principles and diverse operational modes of Atomic Force Microscopy (AFM).
  • It showcases AFM's successful applications in protein research, covering imaging, adsorption, folding-unfolding, assembly, and single molecular recognition.
  • The review emphasizes AFM's capability for in situ, real-time analysis of proteins under near-physiological conditions.

Impact:

  • AFM provides unprecedented insights into protein structure and dynamics at the molecular level.
  • It facilitates a deeper understanding of protein behavior in biologically relevant environments.
  • The technology is poised to drive future innovations in protein science and related fields.