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Updated: Jun 10, 2026
Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis
Published on: October 26, 2015
Construction of protein-modified TiO2 nanoparticles for use with ultrasound irradiation in a novel cell injuring
Chiaki Ogino1, Naonori Shibata, Ryosuke Sasai
1The Division of Material Sciences, Graduate School of Neutral Science and Technology, Kanazawa University, Kakuma, Kanazawa, Japan.
Abstract:
Recently, our group discovered an alternative titanium dioxide (TiO(2)) activation method that uses ultrasound irradiation (US/TiO(2)) instead of ultraviolet irradiation. The pre-S1/S2 protein from hepatitis B virus, which recognizes liver cells, was immobilized to the surface of TiO(2) nanoparticles using an amino-coupling method. The ability of the protein-modified TiO(2) nanoparticles to recognize liver cells was confirmed by surface plasmon resonance analysis and immuno-staining analyses. After uptake of TiO(2) nanoparticles by HepG2 cancer cells, the cells were injured using this US/TiO(2) method; significant cell injury was observed at an ultrasound irradiation intensity of 0.4 W/cm(2). Together with these results, this strategy could be applied to new cell injuring systems that use ultrasound irradiation in place of photodynamic therapy in the near future.
