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Updated: Jun 10, 2026
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
Published on: June 11, 2019
[Differentiation of 5-azacytidine-induced bone marrow stromal stem cells transduced with hepatocyte growth factor
Qian Wen1, Chao-ying Zhou, Ming-qian Zhou
1Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
Objective:
To examine the differentiation of 5-azacytidine-induced bone marrow stromal stem cells (BMSCs) into cardiomyocyte-like cells and hepatocyte growth factor (HGF) gene expression after HGF gene transfection.
Methods:
5-azacytidine was used to induce beagle dog BMSCs to differentiate into cardiomyocyte-like cells. Morphological observation and immunohistochemistry were performed to detect the expression of the markers of cardiomyocyte-like cells including beta-myosin heavy chain (beta-MHC) and alpha-sarcomeric actin. The cells were then transfected with Ad-HGF, and the mRNA and protein expressions of HGF gene were detected by RT-PCR and ELISA, respectively.
Results:
After 4 weeks of induction with 5-azacytidine, the BMSCs differentiated into cardiomyocyte-like cells. The expressions of HGF at the mRNA and protein levels were confirmed in the cells after transfection with Ad-HGF. The peak HGF protein secretion was 10(3) ng/ml at 48 h after transfection.
Conclusion:
Ad-HGF can efficiently transfect BMSCs induced with 5-azacytidine, and this result provides basic experimental evidence for biotherapy of ischemic heart disease using BMSCs.
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