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Updated: Jun 10, 2026

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
Published on: August 26, 2021
[Down-regulation of CD147 expression induces SiHa cell apoptosis through the Bcl-2 pathway]
Xi Zhou1, Jie-yu Lv, Bao-lan Gong
1Department of Obstetrics and Gynecology, People's Hospital Affiliated to Yunyang Medical College, Shiyan 442000, China. xzho98@yahoo.com.cn
Objective:
To investigate the changes in SiHa cell apoptosis after inhibition of CD147 expression.
Methods:
RNA interference (RNAi) technique was used to down-regulate CD147 expression in SiHa cells, and RT-PCR and Western blotting were used to detect expression of CD147, Bcl-2, Bim and caspase-3; the percentage of cell apoptosis were detected by flow cytometry.
Results:
SiRNA sequence 1, 2 inhibited CD147 expression in SiHa cells effectively (P<0.05), resulting also in down-regulated expression of Bcl-2 (P<0.05) and up-regulated expression of caspase-3 and Bim(P<0.05). The percentage of apoptotic cells increased significantly, and early apoptosis was the most obvious in the cells (P<0.05).
Conclusion:
Silencing of CD147 expression induces SiHa cell apoptosis partially through the Bcl-2 pathway .
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