KRAS and BRAF mutation analysis from liquid-based cytology brushings of colorectal carcinoma in comparison with formalin-fixed, paraffin-embedded tissue
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Published on: October 8, 2015
Aris Spathis1, John Georgoulakis, Periklis Foukas
1Department of Cytopathology, University General Hospital Attikon, School of Medicine, National and Kapodistrian University of Athens, Rimini 1, 12462, Chaidari, Greece.
Abstract:
Real-time PCR-based molecular diagnostic techniques were used for the comparative identification of the most common KRAS and BRAF mutations from methanol liquid-based cytology samples and matched, formalin-fixed and paraffin-embedded tissue samples. Methanol-based fixation and preservation of brushing samples of colorectal carcinomas could contribute to sample collection, short-term storage and subsequent molecular analysis, by improving workflow and enhancing the identification of putative mutations.
