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Updated: Jun 10, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Published on: July 7, 2020
Sequence-selective assembly of tweezer molecules on linear templates enables frameshift-reading of sequence
Zhixue Zhu1, Christine J Cardin, Yu Gan
1Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6AD, UK.
Abstract:
Information storage and processing is carried out at the level of individual macromolecules in biological systems, but there is no reason, in principle, why synthetic copolymers should not be used for the same purpose. Previous work has suggested that monomer sequence information in chain-folding synthetic copolyimides can be recognized by tweezer-type molecules binding to adjacent triplet sequences, and we show here that different tweezer molecules can show different sequence selectivities. This work, based on (1)H NMR spectroscopy in solution and on single-crystal X-ray analysis of tweezer-oligomer complexes in the solid state, provides the first clear-cut demonstration of polyimide chain-folding and adjacent-tweezer binding. It also reveals a new and entirely unexpected mechanism for sequence recognition, which, by analogy with a related process in biomolecular information processing, may be termed 'frameshift-reading'. The ability of one particular tweezer molecule to detect, with exceptionally high sensitivity, long-range sequence information in chain-folding aromatic copolyimides is readily explained by this novel process.
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