Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cholinesterases: Distribution and Function01:22

Cholinesterases: Distribution and Function

Cholinesterases are a group of serine hydrolase enzymes that play a crucial role in the breakdown of choline esters. The two primary types of cholinesterases are acetylcholinesterases (AChEs) and butyrylcholinesterase (BuChEs), which differ in their distribution, function, and substrate specificity. AChEs, also known as true cholinesterases, specifically hydrolyze acetylcholine, while BuChEs, often referred to as pseudocholinesterases, can hydrolyze various choline esters, including...
Synthesis of Phosphatidylcholine in the ER Membrane01:27

Synthesis of Phosphatidylcholine in the ER Membrane

The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
The major components of all eukaryotic cell...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Cholinergic Neurons: Neurotransmission01:23

Cholinergic Neurons: Neurotransmission

Cholinergic neurotransmission involves the synthesis and the release of acetylcholine (ACh) in order to transmit nerve impulses across the synapse. The process begins with the synthesis of acetyl CoA, a precursor for ACh, from ATP, acetate, and coenzyme A in the mitochondria. Choline, another vital precursor, is transported inside the neuron through choline transporters, including high-affinity choline transporter CHT1, low-affinity choline transporter CTL1, and lower-affinity choline...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic screening reveals phospholipid metabolism as a key regulator of the biosynthesis of the redox-active lipid coenzyme Q.

Redox biology·2021
Same author

Lipid molecular timeline profiling reveals diurnal crosstalk between the liver and circulation.

Cell reports·2021
Same author

The study of bending properties of monolayer MoS<sub>2</sub> in non-collinear electrodes using first principles theory.

Physical chemistry chemical physics : PCCP·2020
Same author

Implication of phosphatidylethanolamine N-methyltransferase in adipocyte differentiation.

Biochimica et biophysica acta. Molecular basis of disease·2020
Same author

Phospholipid methylation regulates muscle metabolic rate through Ca<sup>2+</sup> transport efficiency.

Nature metabolism·2020
Same author

Hepatic PEMT activity mediates liver health, weight gain, and insulin resistance.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2019
Same journal

Reply to the discussion of "Efficacy of lactoferrin supplementation in pediatric infections: a systematic review and metanalysis".

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

Discussion of "Efficacy of lactoferrin supplementation in pediatric infections: a systematic review and meta-analysis".

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

Does the beta subunit of mitochondrial trifunctional protein modulate monolysocardiolipin acylation to cardiolipin?

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

Distinct effects of disease-associated mutations in the proximal C-linker on negatively cooperative binding of cyclic nucleotides to the human HCN4 channel.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

PPP2R2B targets the JAK2-STAT3 signaling pathway to regulate ferroptosis in breast cancer cells.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

Suppression of CBP/p300 induces growth inhibition and autophagy of hepatocellular carcinoma cells through regulation of EPHA2 by its super-enhancer acetylation.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
See all related articles

Related Experiment Video

Updated: Jun 10, 2026

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
11:23

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

Choline kinase and its function.

Gengshu Wu1, Dennis E Vance

  • 1The Group on the Molecular and Cell Biology of Lipids and the Department of Biochemistry, University of Alberta, Edmonton, AB, Canada.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|July 24, 2010
PubMed
Summary
This summary is machine-generated.

Choline kinase (CK) is crucial for phosphatidylcholine biosynthesis. Gene disruption reveals its unexpected roles in muscular dystrophy, bone deformities, and cancer, suggesting potential therapeutic targets.

More Related Videos

Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
11:11

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

Related Experiment Videos

Last Updated: Jun 10, 2026

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
11:23

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
11:11

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Choline kinase (CK) was discovered in 1953, with significant progress following its 1984 purification.
  • Two genes, Chka and Chkb, encode CK, resulting in three identified isoforms: CKalpha-1, CKalpha-2, and CKbeta.
  • The active enzyme functions as a hetero- or homo-dimer.

Purpose of the Study:

  • To elucidate the physiological roles of choline kinase beyond its known function in phosphatidylcholine biosynthesis.
  • To investigate the consequences of choline kinase gene disruption in vivo.
  • To explore the potential implications of CK in disease pathologies such as muscular dystrophy and cancer.

Main Methods:

  • Gene disruption in mice to create knockout models for Chka and Chkb.
  • Analysis of embryonic development and adult phenotypes in gene-disrupted mice.
  • Biochemical analysis of phosphatidylcholine biosynthesis and catabolism in affected tissues.

Main Results:

  • Mice lacking Chka (CKalpha) exhibit embryonic lethality.
  • Mice lacking Chkb (CKbeta) survive to adulthood but develop hindlimb muscular dystrophy and forelimb bone deformities.
  • Hindlimb muscular dystrophy is linked to altered phosphatidylcholine metabolism, specifically decreased biosynthesis and increased catabolism.

Conclusions:

  • Choline kinase plays critical, previously unrecognized roles in skeletal muscle and bone development.
  • CK dysregulation is implicated in the pathogenesis of muscular dystrophy and potentially cancer.
  • Targeting CK offers a potential therapeutic strategy for certain cancers and possibly other diseases.