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Nonuniform volume changes during muscle contraction.

I R Neering1, L A Quesenberry, V A Morris

  • 1School of Physiology and Pharmacology, University of NSW Kensington, Australia.

Biophysical Journal
|April 1, 1991
PubMed
Summary
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Skeletal muscle fibers dynamically change volume during contraction, with unequal adjustments along the cell. These findings highlight the role of cytoskeletal elements in maintaining force equilibrium in living muscle.

Area of Science:

  • Muscle physiology
  • Cell biology
  • Biophysics

Background:

  • Understanding the mechanical behavior of muscle fibers is crucial for comprehending locomotion and physiological function.
  • Previous studies have primarily focused on external force measurements, with limited insight into internal cellular volume dynamics during contraction.

Purpose of the Study:

  • To investigate dynamic volume changes within intact frog skeletal muscle fibers during isometric tetanic contractions.
  • To correlate these volume alterations with cellular mechanics and cytoskeletal activity.

Main Methods:

  • Utilized a high-speed, intensified, digital-imaging microscope to capture optical cross-sections of live frog muscle fibers.
  • Compared cellular dimensions during resting state with those at the plateau of isometric tetanic contractions.

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Main Results:

  • Observed an increase in volume in the central third of muscle fibers during maximal, constant axial force when the segment was stationary or slightly lengthening.
  • Demonstrated that volume changes were unequal along the fiber axis and not predictable by initial cell shape or area.
  • Identified rapid, localized adjustments within the cytoskeleton potentially responsible for maintaining force equilibrium.

Conclusions:

  • Dynamic, non-uniform volume changes occur within skeletal muscle fibers during contraction.
  • The cytoskeleton plays a critical role in managing these internal adjustments to maintain force balance.
  • Nonuniform volume changes can potentially distort optical measurements in cellular studies.