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Published on: October 16, 2017
Extraction and deproteinization of mannan oligosaccharides
Gang L Huang1, Qin Yang, Zhong B Wang
1College of Chemistry, Chongqing Normal University, Chongqing 400047, China. huangdoctor226@163.com
Summary
Researchers studied mannan oligosaccharide extraction from yeast cell walls. The hydrochloric acid method effectively removed proteins with minimal mannan oligosaccharide loss, making it a promising technique.
Area of Science:
- Biochemistry
- Food Science
Background:
- Mannan oligosaccharides (MOS) are valuable components of yeast cell walls.
- Efficient extraction and purification are crucial for their applications.
Purpose of the Study:
- To compare different deproteinization methods for MOS extraction.
- To evaluate the effectiveness and yield loss associated with each method.
Main Methods:
- Extraction of crude MOS using the dilute alkali-Sevage method.
- Comparison of Sevage, trichloroacetic acid, and hydrochloric acid methods for deproteinization.
- Quantification of deproteinization efficiency and MOS loss.
Main Results:
- Hydrochloric acid method achieved the highest deproteinization percentage.
- Mannan oligosaccharide loss was only slightly higher with the hydrochloric acid method compared to others.
- All tested methods showed varying degrees of deproteinization and associated MOS loss.
Conclusions:
- The hydrochloric acid method is a viable option for deproteinizing MOS extracts.
- Optimizing deproteinization is key to maximizing MOS yield and purity.
- Further research can refine these methods for industrial applications.
