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Related Experiment Videos

Association in normal human fibroblasts of elevated levels of adenosine 3':5'-monophosphate with a selective decrease

B J Baum, J Moss, S D Breul

    The Journal of Biological Chemistry
    |May 25, 1978
    PubMed
    Summary

    Elevated cyclic AMP (adenosine monophosphate) levels in human fibroblasts significantly reduce collagen production. This suggests cyclic AMP regulates the differentiated state of cells involved in collagen synthesis.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Molecular Biology

    Background:

    • Extracellular mediators can influence cellular functions.
    • Cyclic AMP (adenosine monophosphate) is a key intracellular second messenger.
    • Mesenchymal cells, like fibroblasts, are crucial for tissue repair and collagen synthesis.

    Purpose of the Study:

    • To investigate the role of cyclic AMP in regulating collagen production by human fibroblasts.
    • To test the hypothesis that extracellular mediators affect collagen synthesis through a cyclic AMP-dependent mechanism.

    Main Methods:

    • Normal human fibroblasts were incubated with agents known to increase intracellular cyclic AMP (prostaglandin E1, isoproterenol, cholera toxin).
    • Collagen production was assessed by measuring its percentage of total protein synthesis.

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  • Cultures were analyzed for the presence of active collagenase.

    • Main Results:

    • Agents that elevated cyclic AMP levels caused a significant, average 47% reduction in collagen synthesis.
    • This reduction in collagen synthesis occurred with minimal impact on other major extracellular proteins.
    • No active collagenase was detected in the treated fibroblast cultures.

    Conclusions:

    • Intracellular cyclic AMP levels play a significant role in modulating collagen production in normal human fibroblasts.
    • Cyclic AMP may regulate the differentiated state of fibroblasts concerning collagen synthesis.
    • These findings highlight a potential mechanism for controlling collagen deposition in connective tissues.