Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system
Benedetta Peruzzi1, Martina Serra, Chiara Pescucci
1Laboratory of Genetics and Gene Transfer, Core Research Laboratory, Istituto Toscano Tumori (ITT), AOU Careggi, Firenze, Italy.
Molecular and Cellular Probes
|July 27, 2010
Abstract:
Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 'slow' and 'fast' alleles by a single tube reaction.
