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Malignant lymphoma in pleural effusions: an immunocytochemical cell surface analysis
J Guzman1, K J Bross, U Costabel
1Pathologisches Institut der Universitaet, Freiburg, Federal Republic of Germany.
Diagnostic Cytopathology
|January 1, 1991
Summary
Immunocytochemical analysis of pleural effusions aids in identifying lymphoma cells, including B-cell non-Hodgkin
Area of Science:
- Hematology
- Oncology
- Immunology
Background:
- Malignant pleural effusions can be challenging to diagnose.
- Lymphoma subtypes present unique diagnostic profiles.
Purpose of the Study:
- To evaluate the utility of immunocytochemistry in diagnosing lymphoma in malignant pleural effusions.
- To characterize cell surface antigen expression in various lymphoma subtypes within pleural effusions.
Main Methods:
- Analysis of nine malignant pleural effusions using peroxidase-antiperoxidase immunocytochemistry.
- Utilized a broad panel of monoclonal antibodies to detect cell surface antigens.
- Examined lymphoma subtypes including hairy-cell leukemia, B-cell non-Hodgkin's lymphoma, Hodgkin's disease, and plasmacytoma.
Main Results:
- B-cell lymphomas showed high percentages of kappa-positive B cells.
- Hairy cells reacted with CD20, CD25, HLA-DR, CD45, and HLA-1.
- Hodgkin cells reacted with CD15, CD20, CD25, CD30, Tü9, and OKT9.
- Plasmacytoma cells exhibited specific antigen profiles including lambda positivity.
Conclusions:
- Immunocytochemistry effectively identifies B lymphoma, Hodgkin, and hairy cells in pleural effusions.
- Non-malignant lymphocyte subsets in these effusions are similar to other effusions.
- This technique offers clear recognition of malignant cells in pleural fluid.
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