Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hypoxia01:23

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Bone Disorders01:29

Bone Disorders

Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Diabetic Ketoacidosis ll: Pathophysiology01:22

Diabetic Ketoacidosis ll: Pathophysiology

Diabetic ketoacidosis (DKA) is a metabolic emergency characterized by hyperglycemia, ketonemia, and metabolic acidosis. It results from severe insulin deficiency and an excess of counterregulatory hormones, leading to uncontrolled lipolysis, ketogenesis, and widespread electrolyte and fluid disturbances.Pathophysiology The central event in DKA is a profound loss of insulin action. Without insulin, glucose uptake in insulin-dependent tissues is impaired, while hepatic glucose production...
Diagnosing Acidosis and Alkalosis01:24

Diagnosing Acidosis and Alkalosis

Diagnosing acid-base imbalances involves systematically analyzing arterial blood samples, focusing on three key measurements: pH, bicarbonate (HCO3−) concentration, and carbon dioxide partial pressure (PCO2). This analysis follows a four-step process that helps identify the imbalance's underlying cause and nature.
First, the pH level is assessed to determine whether the blood pH is normal (7.35–7.45), low (acidosis), or high (alkalosis).
Next, the PCO2  and HCO3−  values are examined to...
What is the Skeletal System?01:02

What is the Skeletal System?

Overview
Skeleton and Calcium Homeostasis01:21

Skeleton and Calcium Homeostasis

Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Hypoxia mimetics restore bone biomineralisation in hyperglycaemic environments.

Scientific reports·2025
Same author

Extracellular pH is a critical regulator of osteoclast fusion, size and activation.

Bone·2025
Same author

A Machine Learning-Based Image Segmentation Method to Quantify In Vitro Osteoclast Culture Endpoints.

Calcified tissue international·2023
Same author

Evidence that pyrophosphate acts as an extracellular signalling molecule to exert direct functional effects in primary cultures of osteoblasts and osteoclasts.

Bone·2023
Same author

Editorial: Special issue on purinergic signalling and calcification.

Purinergic signalling·2023
Same author

Modulation of osteoblast differentiation and function by the P2X4 receptor.

Purinergic signalling·2022

Related Experiment Video

Updated: Jun 10, 2026

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
09:43

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Published on: March 1, 2022

Acidosis, hypoxia and bone.

Timothy R Arnett1

  • 1Department of Cell & Developmental Biology, University College London, London WC1E 6BT, UK. t.arnett@ucl.ac.uk

Archives of Biochemistry and Biophysics
|July 27, 2010
PubMed
Summary
This summary is machine-generated.

Local pH and oxygen levels significantly impact bone health. Acidosis and hypoxia disrupt bone formation and increase resorption, affecting bone homeostasis.

More Related Videos

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
11:11

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Published on: May 19, 2019

Related Experiment Videos

Last Updated: Jun 10, 2026

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
09:43

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Published on: March 1, 2022

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
11:11

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Published on: May 19, 2019

Area of Science:

  • Bone biology
  • Skeletal physiology
  • Cellular microenvironment

Background:

  • Bone homeostasis is influenced by local pH and oxygen.
  • The skeleton stores alkaline minerals like hydroxyapatite for buffering.
  • Bone cells are sensitive to pH and oxygen levels.

Purpose of the Study:

  • To investigate the effects of pH and oxygen tension on bone cells.
  • To understand the mechanisms of pH and oxygen sensing in bone.
  • To explore the implications for bone disturbances in various diseases.

Main Methods:

  • Review of existing literature on bone cell responses to pH and oxygen.
  • Analysis of cellular mechanisms involved in sensing microenvironmental changes.
  • Correlation of in vitro findings with in vivo conditions and disease states.

Main Results:

  • Acidosis inhibits osteoblast activity but stimulates osteoclast resorption.
  • Hypoxia impairs osteoblast growth and differentiation while promoting osteoclast formation.
  • Osteoclast resorptive function remains unaffected by hypoxia.
  • In vivo, hypoxia often coincides with acidosis, exacerbating negative bone impacts.

Conclusions:

  • Local acidosis and hypoxia negatively affect bone by altering osteoblast and osteoclast activity.
  • Disrupted blood supply, leading to reduced oxygen and pH, has a multifaceted detrimental effect on bone.
  • These findings offer insights into bone pathologies associated with aging, inflammation, and various systemic diseases.