Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 10, 2026

Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy

Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy

Published on: April 17, 2020

Introducing a mouse model of brain death.

G Pomper¹, K Trescher, D Santer

¹Ludwig Boltzmann Cluster for Cardiovascular Research, C/O Core Unit for Biomedical Research, Medical University of Vienna, Vienna General Hospital, Währinger Gürtel 18-20, 1090 Vienna, Austria. gabriela.pomper@meduniwien.ac.at

Journal of Neuroscience Methods

|July 27, 2010

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quantum paraelectric varactors for radiofrequency measurements at millikelvin temperatures.

Nature electronics·2024

Same author

Strong coupling between a microwave photon and a singlet-triplet qubit.

Nature communications·2024

Same author

Current practice in nutrition after allogeneic hematopoietic stem cell transplantation - Results from a survey among hematopoietic stem cell transplant centers.

Clinical nutrition (Edinburgh, Scotland)·2021

Same author

P-Band Induced Self-Organization and Dynamics with Repulsively Driven Ultracold Atoms in an Optical Cavity.

Physical review letters·2019

Same author

Responses to genotoxicity in mouse testicular germ cells and epididymal spermatozoa are affected by increased age.

Toxicology letters·2019

Same author

Near-infrared optical investigations of snow, ice, and water layers on diffuse reflecting surfaces.

The Review of scientific instruments·2019

Researchers developed a reproducible mouse model for brain death (BD) using intracranial pressure (ICP) monitoring. Both rapid and gradual BD induction methods proved successful, offering a standardized tool for transplant research.

Area of Science:

Neuroscience
Experimental Medicine
Transplantation Research

Background:

Experimental animal models are crucial for studying brain death (BD) and its implications in transplantation.
Existing models often involve increasing intracranial pressure (ICP) using balloon-catheters.
Standardization and reproducibility are key for advancing research in this field.

Purpose of the Study:

To develop and validate an experimental mouse model for inducing brain death (BD).
To compare the outcomes of rapid (explosive) versus gradual BD induction under ICP monitoring.
To establish a reliable model for future transplant-associated studies.

Main Methods:

BD was induced in female OF-1 mice using an intracranial balloon-catheter.

More Related Videos

Mouse Cardiac Arrest Model for Brain Imaging and Brain Physiology Monitoring During Ischemia and Resuscitation

Mouse Cardiac Arrest Model for Brain Imaging and Brain Physiology Monitoring During Ischemia and Resuscitation

Published on: April 14, 2023

Study of Experimental Organ Donation Models for Lung Transplantation

Study of Experimental Organ Donation Models for Lung Transplantation

Published on: March 15, 2024

Related Experiment Videos

Last Updated: Jun 10, 2026

Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy

Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy

Published on: April 17, 2020

Mouse Cardiac Arrest Model for Brain Imaging and Brain Physiology Monitoring During Ischemia and Resuscitation

Mouse Cardiac Arrest Model for Brain Imaging and Brain Physiology Monitoring During Ischemia and Resuscitation

Published on: April 14, 2023

Study of Experimental Organ Donation Models for Lung Transplantation

Study of Experimental Organ Donation Models for Lung Transplantation

Published on: March 15, 2024

Two methods were compared: rapid ICP increase (BD ex) and gradual ICP increase (BD grad).

Electroencephalogram (EEG), ICP, and heart rate (HR) were continuously monitored; BD criteria included flat-line EEG, cessation of respiration, and fixed pupils.

Main Results:

Both rapid and gradual BD induction resulted in comparable ICP levels at the time of BD.
Heart rates initially increased post-BD, then decreased in both groups.
The rapid BD induction group showed a significant decrease in HR compared to controls.

Conclusions:

A standardized and reproducible mouse model for inducing brain death has been successfully developed.
The model allows for controlled ICP elevation and comprehensive physiological monitoring.
This validated model is suitable for further investigations in transplantation and related research areas.