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An MRI-based atlas and database of the developing mouse brain
Nelson Chuang1, Susumu Mori, Akira Yamamoto
1Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Neuroimage
|July 27, 2010
Summary
This study presents diffusion tensor imaging (DTI) atlases of developing mouse brains, offering a valuable reference for neuroscience research. These DTI atlases provide high-resolution 3D images for quantitative analysis of brain anatomy in mouse models.
Area of Science:
- Neuroscience
- Developmental Biology
- Medical Imaging
Background:
- Mammalian gene engineering and genetically modified mouse models necessitate advanced tools for mouse brain anatomy analysis.
- Existing anatomical references like histology and MRI atlases have limitations in providing comprehensive developmental data.
Purpose of the Study:
- To create DTI-based anatomical atlases of ex vivo C57BL/6 mouse brains across developmental stages.
- To provide a 3D, high-resolution reference for quantitative analysis of developing mouse brain neuroanatomy.
- To complement existing anatomical resources for neuroscience research.
Main Methods:
- Diffusion Tensor Imaging (DTI) was employed for quantitative characterization of anatomical phenotypes.
- Large deformation diffeomorphic metric mapping was used to create population average images.
- Segmentation of major gray matter and white matter structures was performed.
Main Results:
- DTI-based atlases of ex vivo mouse brains at several developmental stages were generated.
- Distinct tissue contrasts and segmentations of brain structures were achieved.
- Quantitative measurements demonstrated the developmental trajectories of major gray and white matter structures.
Conclusions:
- The developed DTI atlases and database serve as a crucial resource for referencing and analyzing mouse brain anatomy.
- These resources enhance the examination of neuroanatomy in normal, genetically engineered, and disease models.
- The study facilitates a deeper understanding of brain development and anatomical variations in mice.
