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Related Concept Videos

Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon towards...
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...

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Related Experiment Video

Updated: Jun 10, 2026

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
11:39

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

Cell migration: MIM takes the driver's seat.

Véronique Van de Bor1, Stéphane Noselli

  • 1Institute of Developmental Biology & Cancer UMR6543/CNRS, University of Nice Sophia-Antipolis, Parc Valrose, 06108 Nice cedex 2, France.

Current Biology : CB
|July 27, 2010
PubMed
Summary

The I-BAR protein MIM guides cell migration by preventing excessive endocytosis. MIM sequesters cortactin, moderating intracellular signaling of guidance cues.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell migration is crucial for development and disease.
  • Intracellular signaling pathways regulate cell movement.
  • Endocytosis plays a role in signal termination.

Purpose of the Study:

  • To investigate the function of the I-BAR protein MIM in cell migration.
  • To elucidate the mechanism by which MIM influences intracellular signaling.
  • To identify MIM's interaction partners in the context of cell guidance.

Main Methods:

  • Immunofluorescence microscopy to visualize MIM and cortactin localization.
  • Biochemical assays to study protein-protein interactions.
  • Cell migration assays to assess the impact of MIM on cell movement.

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In vitro Cell Migration and Invasion Assays
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In vitro Cell Migration and Invasion Assays

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Study of Cell Migration in Microfabricated Channels
09:36

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

Related Experiment Videos

Last Updated: Jun 10, 2026

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
11:39

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

Study of Cell Migration in Microfabricated Channels
09:36

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

Main Results:

  • MIM exhibits anti-endocytic activity, limiting the internalization of guidance cue receptors.
  • MIM directly interacts with and sequesters cortactin.
  • This sequestration by MIM modulates intracellular signaling downstream of guidance cues, thereby controlling cell migration speed and direction.

Conclusions:

  • MIM plays a conserved role in guiding cell migration through anti-endocytic activity.
  • MIM's sequestration of cortactin is a key mechanism for moderating intracellular signaling.
  • Targeting MIM-cortactin interactions could offer new strategies for controlling cell migration in disease contexts.