Related Experiment Video
Updated: Jun 10, 2026
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Published on: September 20, 2016
Characterization of a family GH5 xylanase with activity on neutral oligosaccharides and evaluation as a pulp
Oscar Gallardo1, María Fernández-Fernández, Cristina Valls
1Department of Microbiology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain.
Abstract:
A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for methyl-glucuronic acid side chains for catalysis. The enzyme was evaluated on Eucalyptus kraft pulp, showing its effectiveness as a bleaching aid.
